Protocol explores chemerin's role in enhancing microglial phagocytosis via the ChemR23/p38 MAPK pathway in cerebral ischemia-reperfusion injury, suggesting neuroprotective potential.
Chemerin 15 (C15), a chemerin-derived peptide, enhances microglial phagocytosis through the ChemR23/p38 MAPK pathway to alleviate brain damage after cerebral ischemia-reperfusion (I/R) injury. Here, we present a protocol for studying C15-enhanced microglial phagocytosis in cerebral I/R injury via the ChemR23/p38 MAPK pathway. We describe steps for establishing in vivo and in vitro models, detecting microglial phagocytic activity, verifying the underlying signaling pathway, and evaluating therapeutic effects. We detail procedures for model construction, functional detection, molecular mechanism verification, and outcome assessment. For complete details on the use and execution of this protocol, please refer to Yang et al.1.
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Mao et al. (2026) studied this question.
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