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July 26, 2026WaterOpen Access

Mechanistic Insights from C/N Ratio and Biodegradability on Methane Yield and Microbial Dynamics in High-Solids Anaerobic Digestion

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Randomized trial investigates methane yield in high-solid anaerobic digestion, highlighting substrate effects.

To investigate the impact of carbon-to-nitrogen ratio and biodegradability on methane yield and microbial dynamics during high-solid anaerobic digestion.
Substrate composition adjusted to achieve varying C/N ratios (2.82–82.72) and biodegradability levels (refractory and easily degraded).
Methane yield measured for different substrates, including white meat, with analysis of total ammonia nitrogen and volatile fatty acids.
Microbial composition differences assessed in relation to changes in C/N ratio and biodegradability.
Highest methane yield of 273.02 mL/g-VS achieved with white meat, 2.22 times higher than higher C/N substrates.
Low C/N substrates yielded higher methane production rates and a significant presence of acetic acid as a volatile fatty acid.
Increased C/N ratio decreased total ammonia nitrogen and alkalinity, with p < 0.05 indicating statistical significance.

Zhou et al. (2026) studied this question.

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July 26, 2026WaterOpen Access

View Full Paper

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Authors

Randomized trial investigates methane yield in high-solid anaerobic digestion, highlighting substrate effects.

To investigate the impact of carbon-to-nitrogen ratio and biodegradability on methane yield and microbial dynamics during high-solid anaerobic digestion.
Substrate composition adjusted to achieve varying C/N ratios (2.82–82.72) and biodegradability levels (refractory and easily degraded).
Methane yield measured for different substrates, including white meat, with analysis of total ammonia nitrogen and volatile fatty acids.
Microbial composition differences assessed in relation to changes in C/N ratio and biodegradability.
Highest methane yield of 273.02 mL/g-VS achieved with white meat, 2.22 times higher than higher C/N substrates.
Low C/N substrates yielded higher methane production rates and a significant presence of acetic acid as a volatile fatty acid.
Increased C/N ratio decreased total ammonia nitrogen and alkalinity, with p < 0.05 indicating statistical significance.

Zhou et al. (2026) studied this question.

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