Literature review and experiments reveal metabolic factors influencing adipogenic differentiation in Wharton's jelly MSCs, indicating potential for improved protocols.
Key Points
To explore the impact of metabolic conditions on the adipogenic differentiation of Wharton's jelly MSCs and establish standardized protocols.
Conducted a structured review of 41 studies on adipogenic differentiation protocols for MSCs.
Compared four adipogenic culture conditions with varying glucose and insulin levels in experimental testing.
Utilized machine learning-assisted lipid imaging to quantify differentiation outcomes.
Wharton's jelly MSCs showed delayed adipogenic differentiation compared to bone marrow MSCs under conventional conditions.
Prolonged cultural induction with enhanced glucose and insulin significantly increased lipid accumulation in WJ-MSCs.
High glucose and insulin levels together led to the strongest adipogenic responses across both MSC sources.