What question did this study set out to answer?

To determine whether the elevated resistance of detyrosinated tubulin-enriched microtubules to depolymerization is a direct consequence of tubulin detyrosination.

January 1, 1988The Journal of Cell BiologyOpen Access

Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level.

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Authors

SKSarwar KhawajaThe University of Queensland GGGregg G. GundersenColumbia University JBJ C BulinskiUniversity of Wisconsin–Madison

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Implication

Laboratory study reveals that detyrosinated microtubules exhibit selective depolymerization resistance in cultured cells, suggesting detyrosination alone does not confer microtubule stability.

Key Points

To determine whether the elevated resistance of detyrosinated tubulin-enriched microtubules to depolymerization is a direct consequence of tubulin detyrosination.
Assessed microtubule stability in cultured monkey kidney (TC-7) cells exposed to nocodazole, cold treatment, and dilution-induced depolymerization in detergent-permeabilized models.
Treated permeabilized cell models with pancreatic carboxypeptidase A to enzymatically convert endogenous tyrosinated microtubules to detyrosinated microtubules.
Tested nocodazole resistance in human fibroblasts lacking endogenous subsets of detyrosinated microtubules.
Detyrosinated microtubules displayed greater resistance than tyrosinated microtubules to nocodazole and dilution-induced depolymerization, but cold-induced depolymerization did not differ significantly between subsets.
Carboxypeptidase A-mediated conversion of tyrosinated microtubules into detyrosinated forms did not significantly increase their resistance to dilution-induced depolymerization.
Human fibroblasts demonstrated stable, nocodazole-resistant microtubule populations despite entirely lacking distinct detyrosinated microtubule arrays.

Cite This Study

Khawaja et al. (1988) studied this question.

synapsesocial.com/papers/6a65abe46645693affa73470 https://doi.org/10.1083/jcb.106.1.141

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Implication

Laboratory study reveals that detyrosinated microtubules exhibit selective depolymerization resistance in cultured cells, suggesting detyrosination alone does not confer microtubule stability.

Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level.

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Implication

Key Points

Cite This Study

Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of detyrosination level.

Discussion

Member takes

Implication

Key Points

Cite This Study

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