Key result
Expression of a functional folate receptor allowed PAM 212 mouse keratinocyte cells to grow in 1 nM 5-methyltetrahydrofolate, incorporating approximately 9-fold more [3H]thymidine than controls.
Why the study?
Does the expression of a functional folate receptor allow PAM 212 cells to grow in low concentrations of 5-methyltetrahydrofolate?
Does the expression of a functional folate receptor allow PAM 212 cells to grow in low concentrations of 5-methyltetrahydrofolate?
The folate receptor is essential for the growth of certain cells in low physiological concentrations of 5-methyltetrahydrofolate.
Folate receptor may be essential for keratinocyte growth in low folate; leaves open its role in human tissues.
The folate receptor mediates the uptake of 5-methyltetrahydrofolate in certain cultured cells by a process called potocytosis. When these cells are grown in physiological concentrations of folate, the receptor increases the efficiency of vitamin uptake by 30-fold. We now show that PAM 212 cells, a mouse keratinocyte cell line, are unable to grow in 1 nM 5-methyltetrahydrofolate unless they express a functional folate receptor. These results suggest that under certain conditions, tissue cells in the body may depend on the folate receptor to obtain enough 5-methyltetrahydrofolate for growth.
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Matsue et al. (1992) studied this question. Folate receptor cDNA transfection vs. Vector-transfected cells was evaluated on Cell growth in 1 nM 5-methyltetrahydrofolate ([3H]thymidine incorporation). Expression of a functional folate receptor allowed PAM 212 mouse keratinocyte cells to grow in 1 nM 5-methyltetrahydrofolate, incorporating approximately 9-fold more [3H]thymidine than controls.
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