Randomized trial investigates GGPP production in engineered cyanobacteria, indicating enhanced natural pigment synthesis potential.
Key Points
The aim is to enhance geranylgeranyl pyrophosphate (GGPP) supply in engineered cyanobacteria for improved production of natural pigments and isoprenoid precursors.
Developed a photosynthetic production platform in Synechocystis sp. PCC 6803 by enhancing GGPP via the MEP pathway.
Evaluated engineered mutants ΔP and ΔGP alongside wild-type under various conditions including carbon supplementation and UV radiation.
Analyzed pigment accumulation and GGPP levels across different physiological conditions.
The ΔGP strain showed approximately a twelvefold increase in GGPP content compared to the control under optimal conditions.
Elevated intracellular GGPP levels were consistently observed, particularly under glucose supplementation and UV treatment.