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The aim is to explore how glucose affects different ATP concentrations in islet beta-cells that correlate with insulin release.

Glucose Generates Sub-plasma Membrane ATP Microdomains in Single Islet β-Cells

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The aim is to explore how glucose affects different ATP concentrations in islet beta-cells that correlate with insulin release.
Utilized recombinant targeted luciferases and photon counting imaging for real-time ATP measurement.
Examined free ATP levels in cytosol, mitochondrial matrix, and plasma membrane of MIN6 and primary beta-cells.
Monitored effects of varying extracellular glucose concentrations (3-30 mM) on ATP levels.
Increased glucose levels elevated [ATP] in all domains, with [ATP]m and [ATP]pm showing sustained increases, while [ATP]c increased transiently.
Extracellular Ca2+ was necessary for increases in [ATP]c and [ATP]pm, but not for [ATP]m.
High [K+] enhanced [ATP]pm increases but had little impact on [ATP]c and [ATP]m increases.

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Randomized trial investigates glucose's effect on ATP microdomains in islet beta-cells, suggesting optimization for insulin release.

Kennedy et al. (1999) studied this question.

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