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February 6, 2018Journal of VirologyOpen Access

N-Terminomics TAILS Identifies Host Cell Substrates of Poliovirus and Coxsackievirus B3 3C Proteinases That Modulate Virus Infection

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Key result

Using the TAILS proteomics approach, 72 and 34 new host protein targets of poliovirus and coxsackievirus B3 3C proteinases were identified in HeLa and HL-1 cell lysates, respectively.

Population

HeLa cell and cardiomyocyte HL-1 cell lysates, and virus-infected cells

Comparison

Poliovirus and coxsackievirus B3 3C proteinases… vs Noncleavable mutant proteins; knockdown of…

Design

Preclinical

Authors

JJJulienne JagdeoADAntoine DufourTKThéo Klein

Discussion

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Overview

Identifies candidate antiviral targets in enterovirus infection; leaves open functional validation before therapeutic consideration.

Structured PICO

P
Population
HeLa cell and cardiomyocyte HL-1 cell lysates, and virus-infected cells
E
Exposure
Poliovirus and coxsackievirus B3 (CVB3) 3C proteinases (3C pro s) evaluated using terminal amine isotopic labeling of substrates (TAILS)
C
Comparator
Noncleavable mutant proteins; knockdown of TAILS-identified target proteins
O
Outcome
Identification of host protein targets of poliovirus and CVB3 3C proteinasessurrogate

N-terminomics by TAILS successfully identified novel host targets of enterovirus 3C proteases, revealing cellular pathways modulated to promote virus infection.

Cite This Study

Jagdeo et al. (2018) studied Enterovirus infection (Poliovirus and Coxsackievirus B3). Poliovirus and coxsackievirus B3 3C proteinases was evaluated on Identification of host protein targets. Using the TAILS proteomics approach, 72 and 34 new host protein targets of poliovirus and coxsackievirus B3 3C proteinases were identified in HeLa and HL-1 cell lysates, respectively.

synapsesocial.com/papers/6a69e779ba61fbfa0247fb5bhttps://doi.org/10.1128/jvi.02211-17
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