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July 14, 2004Molecular and Cellular BiologyOpen Access

La Autoantigen Is Necessary for Optimal Function of the Poliovirus and Hepatitis C Virus Internal Ribosome Entry Site In Vivo and In Vitro

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Key result

Depletion of La autoantigen by siRNA or inhibition by a dominant-negative mutant caused a dramatic reduction in poliovirus and HCV IRES function and virus yield.

Population

In vivo and in vitro models of poliovirus and hepatitis C virus (HCV) translation

Design

Preclinical

Authors

MCMauro Costa‐MattioliYSYuri V. SvitkinNSNahum Sonenberg

Discussion

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Overview

La autoantigen may be an antiviral target for HCV and poliovirus; animal data leave open clinical translation.

Structured PICO

P
Population
In vitro and in vivo cell culture study using HeLa cells to investigate the role of La autoantigen in poliovirus and HCV translation.
I
Intervention
Depletion of La autoantigen using small interfering RNA and a dominant-negative mutant of La
O
Outcome
Poliovirus and HCV internal ribosome entry site (IRES) function and 40S ribosomal subunit bindingsurrogate

The La autoantigen is necessary for optimal IRES-dependent translation of poliovirus and HCV RNAs by stimulating 40S ribosomal subunit binding.

Limitations

  • HCV does not grow in cultured cells, necessitating the use of a poliovirus-HCV chimeric model.

Cite This Study

Costa‐Mattioli et al. (2004) studied Poliovirus and Hepatitis C virus translation. La autoantigen depletion (siRNA) or inhibition (LaDN) vs. Control siRNA or empty vector was evaluated on Poliovirus IRES function and virus yield. Depletion of La autoantigen by siRNA or inhibition by a dominant-negative mutant caused a dramatic reduction in poliovirus and HCV IRES function and virus yield.

synapsesocial.com/papers/6a959bf718385f2ccae3d845https://doi.org/10.1128/mcb.24.15.6861-6870.2004
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The La Autoantigen Contains a Dimerization Domain That Is Essential for Enhancing Translation1997 · 93 citations
  2. 2Intracellular Redistribution of Truncated La Protein Produced by Poliovirus 3C <sup>pro</sup> -Mediated Cleavage1999 · 108 citations
  3. 3La autoantigen enhances translation of BiP mRNA2001 · 95 citations
  4. 4A cellular protein that binds to the 5'-noncoding region of poliovirus RNA: implications for internal translation initiation.1989 · 272 citations
  5. 5Cell-Free, De Novo Synthesis of Poliovirus1991 · 374 citations