Key result
A point mutation changing asparagine to aspartate at amino acid 165 in the puff region of VP2 markedly attenuates the ability of Coxsackievirus B3 to induce myocarditis in mice.
Population
Mice and BALB/c monocytes
Comparison
Coxsackievirus B3 with a point mutation vs Wild-type myocarditic H3 variant of CVB3
Design
Preclinical
Authors
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Attenuates CVB3 myocarditis in mice despite replication; leaves open viral epitope roles in human disease.
A specific point mutation in the VP2 puff region of Coxsackievirus B3 is a key determinant of its ability to induce myocarditis and inflammatory cytokine production.
Knowlton et al. (1996) studied Coxsackievirus B3 induced myocarditis. VP2 amino acid 165 mutation (asparagine to aspartate) vs. Wild-type H3 variant of CVB3 was evaluated on Myocarditic potential and tumor necrosis factor alpha secretion. A point mutation changing asparagine to aspartate at amino acid 165 in the puff region of VP2 markedly attenuates the ability of Coxsackievirus B3 to induce myocarditis in mice.
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