April 1, 2002AJP Lung Cellular and Molecular Physiology
Distribution of intranasal instillations in mice: effects of volume, time, body position, and anesthesia
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Discussion
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Implication
Randomized trial quantifies distribution of substances in mice’s URT and LRT, suggesting volume and anesthesia affect outcomes.
Key Points
- This research aims to determine how delivery volume, body position, and anesthesia affect the distribution of substances in the upper and lower respiratory tracts of mice.
- Mice were instilled with a nonabsorbable tracer and then euthanized for gamma scintigraphy analysis.
- Relative distribution was measured at different volumes and under varying conditions of anesthesia.
- The effect of body position on distribution was also examined.
- At a 5 microl delivery volume, no radiation reached the lower respiratory tract (LRT); at 50 microl, distribution to the LRT was 55.7 +/- 2.5%.
- Under anesthesia, 25 microl resulted in 30.1 +/- 6.9% distribution to the LRT; while in awake mice, this was only 5.3 +/- 1.5% (P < 0.05).
- Varying body positions did not impact the distribution of the tracer.
Cite This Study
Southam et al. (2002) studied this question.