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A method is described for propagation of human amnion cells (Strain FL) in Spinner-type culture vessels of volumes up to 3 liter capacity. Under these conditions, it is possible to maintain a stock culture which can supply cells for growth in roller tubes and Povitsky bottles without the necessity of a trypsinization procedure. The elimination of this dispersing procedure saves considerable time in handling of the cells. These cells after being propagated in submerged culture retain their susceptibility to type 4 adenovirus.
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Davis et al. (1958) studied this question.
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