What is the clinical evidence from this study?

Study design: Other. Intervention: Electrical shocks. Primary outcome: Changes in intracellular calcium concentration and membrane potential.

What question did this study set out to answer?

The study aims to investigate how electrical shocks affect intracellular calcium concentration and membrane potential in cardiac myocytes.

May 25, 2004Circulation Research

Effects of Electrical Shocks on Ca i 2+ and V m in Myocyte Cultures

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Key result

Electrical shocks transiently decreased intracellular calcium at sites of both negative and positive membrane potential changes, with very strong shocks causing postshock elevation of diastolic calcium.

Population

Geometrically defined myocyte cultures (cell strands, width=0.8 mm) and an ionic model of adult rat myocytes

Design

Preclinical

Authors

VFVladimir G. FastUniversity of Alabama at Birmingham ECEric R. CheekUniversity of Alabama at Birmingham APAndrew E. PollardUniversity of Alabama at Birmingham

Discussion

Member takes

Implication

Shock-induced ΔCai2+ and ΔVm mapping is feasible in myocyte strands; leaves open their mechanistic role in defibrillation.

Key Points

The study aims to investigate how electrical shocks affect intracellular calcium concentration and membrane potential in cardiac myocytes.
Measured shock-induced changes in intracellular calcium and membrane potential in defined myocyte cultures.
Applied shocks of varying intensities (5 to 40 V/cm) during the action potential plateau.
Used double-staining techniques with Vm-sensitive and Ca(i)2+-sensitive dyes.
Electrical shocks caused a transient decrease in intracellular calcium at both positive and negative membrane potential changes.
High shocks (>20 V/cm) resulted in smaller decreases in intracellular calcium and elevated diastolic calcium, likely due to membrane electroporation.
Nifedipine application eliminated calcium decreases at positive membrane potential sites, highlighting the role of calcium current.

Structured PICO

Population

Geometrically defined myocyte cultures (cell strands, width=0.8 mm) and an ionic model of adult rat myocytes

Intervention

Electrical shocks (E approximately 5 to 40 V/cm) applied during the action potential plateau

Outcome

Changes in intracellular calcium concentration (DeltaCa(i)2+) and corresponding Vm changes (DeltaVm)surrogate

Electrical shocks transiently decrease intracellular calcium at sites of both positive and negative membrane potential changes, with very strong shocks causing membrane electroporation and elevated diastolic calcium.

Cite This Study

Fast et al. (2004) studied this question. Electrical shocks was evaluated on Changes in intracellular calcium concentration and membrane potential. Electrical shocks transiently decreased intracellular calcium at sites of both negative and positive membrane potential changes, with very strong shocks causing postshock elevation of diastolic calcium.

synapsesocial.com/papers/6a6a4afe34b735385cb37b7f https://doi.org/10.1161/01.res.0000132746.94360.8b

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Effects of Electrical Shocks on Ca i 2+ and V m in Myocyte Cultures

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Effects of Electrical Shocks on Ca i 2+ and V m in Myocyte Cultures

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider