Key result
rHVT-IBD vaccination induced cell-mediated immunity responses in specific pathogen-free chickens at 5 weeks, with ELISPOT being more sensitive than ELISA for detecting chicken interferon-gamma.
Why the study?
Does rHVT-IBD vaccination induce cell-mediated immunity and can the VP2 protein/gene be detected in specific pathogen-free chickens?
Does rHVT-IBD vaccination induce cell-mediated immunity and can the VP2 protein/gene be detected in specific pathogen-free chickens?
The study developed tools to detect the VP2 protein and demonstrated that rHVT-IBD vaccination induces cell-mediated immunity in chickens, which can be sensitively measured using an enzyme-linked immunospot approach.
ELISPOT may improve CMI detection sensitivity after rHVT-IBD; leaves open validation and translation beyond SPF chickens.
Infectious bursal disease (IBD) is an avian viral disease that causes severe economic losses in the poultry industry worldwide. The live IBD virus (IBDV) has a potential immunosuppressive effect. Currently available IBDV vaccines have shortcomings, prompting the development of safer and more effective vaccination approaches, including the use of the recombinant turkey herpesvirus vaccine expressing the immunogenic structural VP2 protein of IBDV (recombinant HVT (rHVT)-IBD). The objectives of this study were twofold: (i) to develop in vitro assays and molecular tools to detect the VP2 protein and gene and (ii) to evaluate cell-mediated immunity (CMI) induced by rHVT-IBD vaccination of day-old specific pathogen-free chickens. The VP2 protein expressed by rHVT-IBD-infected chicken embryo fibroblasts was detected using the enzyme-linked immunosorbent assay and immunofluorescence. Using molecular techniques, the VP2 gene was detected in various organs, providing a method to monitor vaccine uptake. rHVT-IBD vaccination induced CMI responses in specific pathogen-free chickens at 5 weeks. CMI was detected by measuring chicken interferon-gamma after ex vivo antigenic stimulation of splenocytes. Moreover, our results showed that the enzyme-linked immunospot approach is more sensitive in detecting chicken interferon-gamma than enzyme-linked immunosorbent assay. The tools developed in this study may be useful in the characterization of new-generation recombinant vaccines and the cellular immune response they induce.
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Ingrao et al. (2016) studied Infectious bursal disease (IBD). recombinant HVT-IBD vaccination was evaluated on Cell-mediated immunity (CMI) responses. rHVT-IBD vaccination induced cell-mediated immunity responses in specific pathogen-free chickens at 5 weeks, with ELISPOT being more sensitive than ELISA for detecting chicken interferon-gamma.
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