What question did this study set out to answer?

To design and validate a 14-color antibody combination for monitoring CD4+ T-cell populations and establish age-related reference values.

February 27, 2020Frontiers in ImmunologyOpen Access

Age Distribution of Multiple Functionally Relevant Subsets of CD4+ T Cells in Human Blood Using a Standardized and Validated 14-Color EuroFlow Immune Monitoring Tube

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Randomized trial examines immune monitoring of CD4+ T-cell subsets in blood samples, suggesting new clinical utility for disease evaluation.

To design and validate a 14-color antibody combination for monitoring CD4+ T-cell populations and establish age-related reference values.
Validated a 14-color antibody combination through extensive testing.
Analyzed 130 blood samples and established age ranges from 0-89 years for reference values.
Evaluated CD4+ T-cell profiles in disease models including MBL, SM, and CVID with 25 additional samples.
Identified ≥89 different CD4+ T-cell populations with high multicenter reproducibility.
Established specific age-related kinetics for naïve T-cells and various Th subsets throughout life.
Detected altered CD4+ T-cell profiles in systemic mastocytosis and common variable immunodeficiency.

Botafogo et al. (2020) studied this question.

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February 27, 2020Frontiers in ImmunologyOpen Access

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Authors

Randomized trial examines immune monitoring of CD4+ T-cell subsets in blood samples, suggesting new clinical utility for disease evaluation.

To design and validate a 14-color antibody combination for monitoring CD4+ T-cell populations and establish age-related reference values.
Validated a 14-color antibody combination through extensive testing.
Analyzed 130 blood samples and established age ranges from 0-89 years for reference values.
Evaluated CD4+ T-cell profiles in disease models including MBL, SM, and CVID with 25 additional samples.
Identified ≥89 different CD4+ T-cell populations with high multicenter reproducibility.
Established specific age-related kinetics for naïve T-cells and various Th subsets throughout life.
Detected altered CD4+ T-cell profiles in systemic mastocytosis and common variable immunodeficiency.

Botafogo et al. (2020) studied this question.

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