Blebbistatin treatment resulted in 80% survival (4/5) in PRRSV-infected piglets compared to 100% mortality in the control group, demonstrating MYH9 is essential for PRRSV infection.
Identification of MYH9 as an essential factor for PRRSV infection provides new insights into viral entry and potential control strategies for this swine disease.
Absolute Event Rate: 80% vs 0%
Porcine reproductive and respiratory syndrome (PRRS) caused by the PRRS virus (PRRSV) is an important swine disease worldwide. PRRSV has a limited tropism for certain cells, which may at least in part be attributed to the expression of the necessary cellular molecules serving as the virus receptors or factors on host cells for virus binding or entry. However, these molecules conferring PRRSV infection have not been fully characterized. Here we show the identification of non-muscle myosin heavy chain 9 (MYH9) as an essential factor for PRRSV infection using the anti-idiotypic antibody specific to the PRRSV glycoprotein GP5. MYH9 physically interacts with the PRRSV GP5 protein via its C-terminal domain and confers susceptibility of cells to PRRSV infection. These findings indicate that MYH9 is an essential factor for PRRSV infection and provide new insights into PRRSV-host interactions and viral entry, potentially facilitating development of control strategies for this important swine disease.
Gao et al. (Tue,) conducted a other in Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection (n=15). Blebbistatin vs. PBS (mock) was evaluated on Survival rate at 21 days post infection. Blebbistatin treatment resulted in 80% survival (4/5) in PRRSV-infected piglets compared to 100% mortality in the control group, demonstrating MYH9 is essential for PRRSV infection.