Key result
Stretch loading of human periodontal ligament fibroblastic cells upregulated TGF-β1 and ALP expression, a process mediated by Angiotensin II via the type 2 receptor.
Why the study?
Does stretch loading and Angiotensin II stimulation increase gene expressions of TGF-β1 and ALP in periodontal ligament cells?
Does stretch loading and Angiotensin II stimulation increase gene expressions of TGF-β1 and ALP in periodontal ligament cells?
Angiotensin II mediates loading signals in stretched periodontal ligament cells to induce expressions of TGF-β1 and ALP via the type 2 receptor.
Stretch-induced PDL gene upregulation is hypothesis-generating; clinical relevance for occlusion or mastication remains undetermined.
The loading caused by occlusion and mastication plays an important role in maintaining periodontal ligament (PDL) tissues. We hypothesized that a loading magnitude would be involved in the production of biological factors that function in the maintenance of PDL tissues. Here, we identified up-regulated gene expressions of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and angiotensinogen in human PDL fibroblastic cells (HPLFs) that were exposed to 8% stretch loading. Immunolocalization of angiotensin I/II (Ang I/II), which was converted from angiotensinogen, was detected in rat PDL tissues. HPLFs that were stimulated by Ang II also increased their gene expressions of TGF-β1 and ALP. Furthermore, the antagonist for Ang II type 2 receptor, rather than for type 1, significantly inhibited gene expressions induced by the stretch loading. Analysis of these data suggests that Ang II mediates the loading signal in stretched HPLFs to induce expressions of TGF-β1 and ALP.
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Monnouchi et al. (2011) studied this question. Stretch loading and Angiotensin II stimulation was evaluated on Gene expressions of TGF-β1, ALP, and angiotensinogen. Stretch loading of human periodontal ligament fibroblastic cells upregulated TGF-β1 and ALP expression, a process mediated by Angiotensin II via the type 2 receptor.
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