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February 1, 1994Journal of Biological ChemistryOpen Access

Angiotensin II stimulates the release of phospholipid-derived second messengers through multiple receptor subtypes in heart cells.

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Key result

Angiotensin II stimulates distinct phospholipases in parallel through AT1 and AT2 receptors in cultured neonatal rat cardiac myocytes.

Population

Cultured neonatal rat cardiac myocytes

Design

Preclinical

Authors

ALAndrew J. LokutaUniversity of Wisconsin–MadisonCCCarlton R. CooperUniversity of DelawareSGS T GaaUniversity of Maryland, Baltimore

Discussion

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Implication

Ang-II–induced ARA release in myocytes is hypothesis-generating; clinical relevance in human cardiac disease remains undetermined.

Key Points

  • This study aims to elucidate the molecular mechanisms through which angiotensin II influences heart cell function and signaling.
  • Cultured neonatal rat cardiac myocytes were stimulated with angiotensin II to assess the release of arachidonic acid and inositol phosphates.
  • Mass spectroscopy and receptor-specific antagonists were utilized to analyze the pathways involved.
  • Phospholipase inhibitors were employed to investigate the role of different enzymes in the signaling process.
  • Ang-II stimulated a sustained release of arachidonic acid, achieving a half-maximal response at 0.1 nM.
  • AT1 receptor antagonists inhibited inositol phosphate accumulation effectively (DuP 753 IC50 approximately 7 nM).
  • AT2 receptor antagonists significantly inhibited arachidonic acid release (CGP 42112A IC50 approximately 7 nM).

Structured PICO

P
Population
Cultured neonatal rat cardiac myocytes
I
Intervention
Angiotensin II (Ang-II) with AT1 and AT2 receptor-specific antagonists and phospholipase inhibitors
O
Outcome
Release of arachidonic acid (ARA) and accumulation of inositol phosphatessurrogate

Angiotensin II activates distinct phospholipase pathways in parallel through AT1 and AT2 receptors in cardiac myocytes.

Cite This Study

Lokuta et al. (1994) studied this question. Angiotensin II was evaluated on Release of arachidonic acid and inositol phosphate accumulation. Angiotensin II stimulates distinct phospholipases in parallel through AT1 and AT2 receptors in cultured neonatal rat cardiac myocytes.

synapsesocial.com/papers/6a6a85c845e10bb5bcf2bdf0https://doi.org/10.1016/s0021-9258(17)37619-6
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Potassium Channels in Cardiac Cells Activated by Arachidonic Acid and Phospholipids1989 · 331 citations
  2. 2Effect of angiotensin II on cytosolic free calcium in neonatal rat cardiomyocytes1991 · 88 citations
  3. 3Angiotensin II increases spontaneous contractile frequency and stimulates calcium current in cultured neonatal rat heart myocytes: insights into the underlying biochemical mechanisms.1988 · 198 citations
  4. 4Induction of Membrane Ruffling and Fluid-Phase Pinocytosis in Quiescent Fibroblasts by ras Proteins1986 · 742 citations
  5. 5Cytosolic phospholipase A2 is coupled to hormonally regulated release of arachidonic acid.1992 · 546 citations