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February 1, 1995VirologyOpen Access

Vpr Is Required for Efficient Replication of Human Immunodeficiency Virus Type-1 in Mononuclear Phagocytes

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Authors

RCRuth I. ConnorDartmouth HealthBCBenjamin K. ChenWorld Wide Web ConsortiumSCSunny ChoeAaron Diamond AIDS Research Center

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Implication

Randomized trial demonstrates Vpr's role in HIV-1 replication in mononuclear phagocytes, indicating new therapeutic targets.

Key Points

  • This research investigates the role of the Vpr protein in the replication of HIV-1 in mononuclear phagocytes.
  • Utilized replication competent, macrophage-tropic HIV-1 and a luciferase HIV-1 reporter vector.
  • Conducted PCR analysis of viral DNA to localize infection block in monocyte/macrophage cells.
  • Tested virions with Vpr molecules produced in complementing cells.
  • Vpr is crucial for efficient replication of HIV-1 in primary monocyte/macrophages.
  • No role for Vpr was observed in activated or resting T cell infection.
  • Infection blockage is after entry and reverse transcription, but before or at proviral transcription.

Cite This Study

Connor et al. (1995) studied this question.

synapsesocial.com/papers/6a6a8fe126cff2e2f6f417f5https://doi.org/10.1006/viro.1995.1016
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Also Consider

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  1. 1HIV-1 Vpr combats the PU.1-driven antiviral response in primary human macrophages2024 · 10 citations
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  5. 5HIV-1 Vpr causes separate cell cycle arrests in G2 and M that activate alternative DNA damage pathways2024 · 3 citations