The PFA-100 analyzer demonstrated 100% sensitivity with collagen-ADP and 96.5% with collagen-epinephrine for detecting von Willebrand disease, superior to the 65.5% sensitivity of bleeding time.
Cross-Sectional (n=185)
Does the PFA-100 analyzer improve the detection of von Willebrand disease compared to bleeding time?
The PFA-100 analyzer demonstrates high sensitivity and specificity for screening von Willebrand disease, significantly outperforming traditional bleeding time tests.
Absolute Event Rate: 100% vs 65.5%
We have evaluated the performance of a new analyzer using high shear stress, the PFA-100 (Platelet Function Analyzer, Dade International, Massy, France), for screening of patients with von Willebrand disease (vWD). Whole citrated blood is aspirated through a capillary to the central aperture of a membrane coated with collagen and with a platelet agonist (either epinephrine or adenosine diphosphate ADP). The time required to obtain occlusion of the aperture by a platelet plug is defined as the closure time (CT). We studied 60 patients with different types of vWD and 96 normal subjects. Fourteen subjects with hemophilia and 15 patients with a platelet disorder were also analyzed. When omitting results from two patients with type 2N, the 58 other patients with type 1, type 2A, type 2B, type 3, or acquired vWD all exhibited an abnormal occlusion with collagen-ADP (sensitivity, 100%) and 56 of 58 had an abnormal CT with collagen-epinephrine (sensitivity, 96.5%). Only two patients with mild type 1 were not detected with collagen-epinephrine. In comparison, the bleeding time (BT) was normal in 20 patients: 17 with type 1, two with type 2A, and one with acquired vWD (sensitivity, 65.5%). The specificity of the PFA-100 was over 95% with both types of cartridges. Thus, the analyzer is well adapted to routine testing, as it has the advantages of simplicity and ease of execution, and demonstrates a high sensitivity, clearly superior to that of BT, for the screening of patients with vWD.
Fressinaud et al. (Sun,) conducted a cross-sectional in von Willebrand disease (n=185). PFA-100 analyzer vs. Bleeding time (BT) was evaluated on Sensitivity for detecting von Willebrand disease (excluding type 2N). The PFA-100 analyzer demonstrated 100% sensitivity with collagen-ADP and 96.5% with collagen-epinephrine for detecting von Willebrand disease, superior to the 65.5% sensitivity of bleeding time.