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September 4, 2001Circulation

Increased Bleeding Tendency and Decreased Susceptibility to Thromboembolism in Mice Lacking the Prostaglandin E Receptor Subtype EP 3

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Key result

Mice lacking the PGE2 receptor subtype 3 (EP3-/-) exhibited significantly prolonged bleeding time and reduced mortality and lung thrombus formation when challenged with arachidonic acid.

Population

Mice lacking the PGE2 receptor subtype 3 (EP3-/- mice) and murine platelets

Comparison

Genetic deletion of EP3 receptor and in vitro… vs Wild-type mice/platelets (implied)

Design

Preclinical

Authors

HMHong MaAHAkiyoshi HaraCXChun Xiao

Discussion

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Overview

EP3 deletion attenuates thrombosis in mice; leaves open whether selective antagonism is antithrombotic in humans.

Structured PICO

P
Population
Experimental study using mice lacking the PGE2 receptor subtype 3 (EP3-/-) to evaluate the role of PGE2 in hemostasis and thromboembolism.
I
Intervention
Genetic deletion of EP3 receptor (EP3-/-) and in vitro exposure to PGE2 and AE-248 (selective EP3 agonist)
C
Comparator
Wild-type mice/platelets (implied)
O
Outcome
Platelet aggregation, bleeding time, mortality, and thrombus formation in the lung after arachidonic acid challengesurrogate

PGE2 potentiates platelet aggregation via the EP3 receptor, and its absence in mice leads to increased bleeding tendency and decreased susceptibility to thromboembolism.

Cite This Study

Ma et al. (2001) studied Hemostasis and thromboembolism. EP3 receptor knockout vs. Wild-type mice was evaluated on Bleeding time, mortality, and thrombus formation. Mice lacking the PGE2 receptor subtype 3 (EP3-/-) exhibited significantly prolonged bleeding time and reduced mortality and lung thrombus formation when challenged with arachidonic acid.

synapsesocial.com/papers/6a6ae6c747a1bfb288f2f6cdhttps://doi.org/10.1161/hc3601.094003
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Blocking the EP3 receptor for PGE2 with DG-041 decreases thrombosis without impairing haemostatic competence2013 · 53 citations
  2. 2Activation of the murine EP3 receptor for PGE2 inhibits cAMP production and promotes platelet aggregation2001 · 173 citations
  3. 3Prostaglandin F2α Facilitates Platelet Activation by Acting on Prostaglandin E2 Receptor Subtype EP3 and Thromboxane A2 Receptor TP in Mice2019 · 15 citations
  4. 4Selective activation of the prostaglandin E2 receptor subtype EP2 or EP4 leads to inhibition of platelet aggregation2010 · 45 citations
  5. 5Vascular wall–produced prostaglandin E2 exacerbates arterial thrombosis and atherothrombosis through platelet EP3 receptors2007 · 180 citations