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May 26, 2019Thrombosis and Haemostasis

Prostaglandin F2α Facilitates Platelet Activation by Acting on Prostaglandin E2 Receptor Subtype EP3 and Thromboxane A2 Receptor TP in Mice

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Key result

PGF2α potentiates platelet aggregation in vitro via EP3 and TP receptors, and facilitates hemostasis and thromboembolism in vivo in mice.

Why the study?

While other prostanoids regulate platelet function, the effect of PGF2alpha on platelet function remained to be determined.

Does Prostaglandin F2α facilitate platelet activation and thromboembolism in mice?

Population

Wild-type mice and mice lacking FP, EP3, TP, or both EP3 and TP

Comparison

PGF2alpha treatment vs control in vitro and in vivo across mouse genotypes

Design

In vitro and in vivo animal experimental study

Authors

HKHitoshi KashiwagiKYKoh-ichi YuhkiYIYoshitaka Imamichi

Discussion

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Overview

PGF2α may promote thromboembolism via EP3/TP; leaves open human relevance and targeted intervention studies.

Structured PICO

Does Prostaglandin F2α facilitate platelet activation and thromboembolism in mice?

P
Population
Mice (wild-type and receptor knockouts) used to study the effect of PGF2α on platelet function in vitro and in vivo.
I
Intervention
Prostaglandin F2α (PGF2α) administered in vitro and in vivo (intravenously)
C
Comparator
Wild-type vs knockout platelets/mice; baseline without PGF2α
O
Outcome
Platelet aggregation in vitro, bleeding time, and arachidonic acid-induced acute thromboembolism in vivosurrogate

PGF2α facilitates platelet aggregation and thromboembolism in vivo by acting on EP3 and TP receptors, rather than its canonical FP receptor.

Cite This Study

Kashiwagi et al. (2019) studied Platelet function and thrombosis. PGF2α was evaluated on Platelet aggregation, bleeding time, and acute thromboembolism. PGF2α potentiates platelet aggregation in vitro via EP3 and TP receptors, and facilitates hemostasis and thromboembolism in vivo in mice.

synapsesocial.com/papers/6a9460bbd1fa461db0c57779https://doi.org/10.1055/s-0039-1688906
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The role of prostanoid receptors in mediating the effects of PGE<sub>2</sub>on human platelet function2010 · 61 citations
  2. 2Activation of the murine EP3 receptor for PGE2 inhibits cAMP production and promotes platelet aggregation2001 · 173 citations
  3. 3Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides.1975 · 3,062 citations
  4. 4Aspirin Half Maximal Inhibitory Concentration Value on Platelet Cyclooxygenase1 in Severe Type-2 Diabetes Mellitus is not Significantly Different from that of Healthy Individuals2013 · 4 citations
  5. 5Atherosclerosis — An Inflammatory Disease1999 · 21,813 citations