Key result
This unit describes methods that permit the biochemical isolation and purification of transverse tubules (T-tubules) from mammalian skeletal muscle.
This paper provides methodological protocols for the isolation and purification of T-tubules from skeletal muscle.
May aid T-tubule studies in cardiac models; leaves open clinical relevance pending validation.
The transverse tubules (T-tubules) of mammalian cardiac and skeletal muscles are invaginations of the sarcolemma. They play a crucial role in excitation-contraction coupling as well as in intracellular signaling and in regulation of glucose transport. The biochemical purification of T-tubule membranes is a difficult task, and membrane fractions enriched in transverse tubules are usually contaminated with other cell-surface and intracellular membranes. This unit includes methods that permit the isolation and purification of T-tubules from skeletal muscle.
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Zorzano et al. (2006) studied this question. Isolation and purification of T-tubules was evaluated. This unit describes methods that permit the biochemical isolation and purification of transverse tubules (T-tubules) from mammalian skeletal muscle.
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