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A radioimmunoassay of trypsin and trypsinogen in fish has been developed. The antibodies detect both trypsin and trypsinogen, and the presence of trypsin inhibitors in the assay extract does not affect the readings. The radioimmunoassay, used in combination with conventional enzyme assay, is very suitable for mechanistic studies of the production and activation of trypsin in fish.
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Hjelmeland et al. (1985) studied this question.
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