What question did this study set out to answer?

Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family.

View Full Paper

Ask AI

Bookmark

Authors

Randomized screening identifies new members of protein-serine kinase family, suggesting novel applications in molecular biology.

This research aims to identify cDNA clones encoding members of the protein-serine kinase family using hybridization techniques.
Screened 80,000 cDNA clones from a HeLa library using mixed oligonucleotide probes.
Identified 19 clones with strong hybridization signals to distinct probes.
Characterized 4 selected clones through partial DNA sequence analysis.
3 out of 4 characterized clones encode typical protein-serine kinases.
One deduced sequence shares 72% identity with rabbit skeletal muscle phosphorylase kinase gamma-subunit.
Another sequence is closely related to yeast kinases CDC2 and CDC28.

Steven K. Hanks (1987) studied this question.

View Full Paper

Ask AI

Bookmark