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Switch the lights off! A novel sensitive enzyme-activity assay combines the advantageous features of upconverting phosphors (UCPs) and fluorescence quenching. The new assay principle allows the use of fluorescent particulate labels in a quenching-based assay despite the fact that it is not possible to achieve adequate quenching efficiency of particle fluorescence directly. Infrared excitation enables even the use of colorful samples.
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Rantanen et al. (2008) studied this question.
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