Key result
Early acclimatization to LHTLH significantly increased sleep disturbance (4.9 vs. 2.5 a.u., p<0.001) and altered sleep architecture and heart rate variability compared to normoxia.
Why the study?
Living High-Training Low and High is used to improve sea-level physical performance, but its effects on sleep, heart rate variability, and psychological stress needed investigation.
Does Living High-Training Low and High (LHTLH) affect sleep, heart rate variability, and psychological stress in elite female cyclists?
Observational (n=10)
Does Living High-Training Low and High (LHTLH) affect sleep, heart rate variability, and psychological stress in elite female cyclists?
May warrant caution during early LHTLH acclimatization in elite female cyclists; leaves open optimal protocols for recovery and performance.
ABSTRACT “Living High‐Training Low and High” (LHTLH) is an altitude/hypoxic training method used to improve physical performance at sea level. The aim of this exploratory study was to investigate the effects of LHTLH on sleep, heart rate variability (HRV), and psychological stress in 10 elite/international level female cyclists (mean age: 17.3 ± 1.2 years). Participants were monitored for 19 days divided into 3 periods: (i) normoxia (5 days preceding LHTLH), (ii) early acclimatization (day 1–4 of LHTLH), and (iii) middle acclimatization (day 5–14) performed in hypoxic rooms (F i O 2 = 15.09%). Questionnaires of psychological stress and sleep disturbance, sleep architecture (determined through an electroencephalography sleep headband), and HRV (measured at rest with a chest strap) were recorded during the 3 periods. The results found that, compared to normoxia, there was no significant difference in total sleep time, sleep efficiency, latency, or waking during the early acclimatization period. However, a significant increase in sleep disturbance (2.5 ± 1.1 vs. 4.9 ± 2.5 a.u. and p < 0.001), alterations of HRV, and sleep architecture with a significant increase in stages 1 (21.8 ± 3 vs. 25.9 ± 3.6 min and p < 0.007) and 2 (201.2 ± 55 vs. 238.5 ± 55 min and p < 0.008) of sleep was observed. During the middle acclimatization period, the athletes had restorative sleep but HRV remained altered, with a significant increase in external tension (1.24 ± 0.4 vs. 2.83 ± 1.8 a.u. and p < 0.05). All these findings suggest that an acclimatization period of at least 4 days is required to recover restorative sleep during LHTLH intervention. This article provides the first insights on the understanding of psychophysiological stress induced using the “Live High‐Training Low and High” paradigm, particularly in elite/international level female athletes. Results indicate that: during the early acclimatization period, sleep disturbances are observed, with an alteration in heart rate variability. After this period, the athletes return to restful sleep, enabling them to increase their training load. However, heart rate variability and psychological stress remain impaired for at least 10 days.
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Pirlot et al. (2025) conducted an observational in Elite/international level female cyclists (n=10). Living High-Training Low and High (LHTLH) vs. Normoxia (5 days preceding LHTLH) was evaluated on Sleep disturbance during early acclimatization (a.u.) (p=<0.001). Early acclimatization to LHTLH significantly increased sleep disturbance (4.9 vs. 2.5 a.u., p<0.001) and altered sleep architecture and heart rate variability compared to normoxia.
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