What question did this study set out to answer?

To determine whether the immunosuppressive compound FTY720 suppresses peripheral immune function by blocking the export of mature T lymphocytes from the thymus.

May 1, 2000European Journal of Immunology

Immunosuppressant FTY720 inhibits thymocyte emigration

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Authors

HYHideki YagiInternational University of Health and Welfare RKRikiya KambaOsaki Citizen Hospital KCKenji ChibaKeio University

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Implication

Animal study demonstrates that FTY720 inhibits thymocyte emigration in mice, suggesting immunosuppression occurs partly via sequestering mature T cells within the thymus.

Key Points

To determine whether the immunosuppressive compound FTY720 suppresses peripheral immune function by blocking the export of mature T lymphocytes from the thymus.
Administered daily doses of FTY720 (1 mg/kg) or saline control to mice and quantified peripheral and thymic lymphocyte subpopulations.
Characterized phenotypic surface markers on thymocytes, including TCRalpha beta, H-2Kd, CD44, CD69, CD24, and L-selectin.
Assessed cellular migration to peripheral lymphoid tissues (spleen and lymph nodes) using intrathymic fluorescein isothiocyanate (FITC) cell labeling.
FTY720 administration markedly depleted circulating peripheral blood T lymphocytes while causing a three- to fourfold increase in mature medullary thymocytes (CD4+CD8- and CD4-CD8+).
Accumulated thymocytes displayed high levels of L-selectin along with markers typical of recent thymic emigrants, reflecting trapped mature cells.
Intrathymic FITC tracking showed that labeled thymocyte migration to the spleen and lymph nodes was reduced to one-fourth (a 75% decrease) of control levels.

Cite This Study

Yagi et al. (2000) studied this question.

synapsesocial.com/papers/6a6bad6e6f523709a024c2ab https://doi.org/10.1002/(sici)1521-4141(200005)30:5<1435::aid-immu1435>3.0.co;2-o

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Implication

Animal study demonstrates that FTY720 inhibits thymocyte emigration in mice, suggesting immunosuppression occurs partly via sequestering mature T cells within the thymus.

Immunosuppressant FTY720 inhibits thymocyte emigration

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Implication

Key Points

Cite This Study

Immunosuppressant FTY720 inhibits thymocyte emigration

Discussion

Member takes

Implication

Key Points

Cite This Study

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