What question did this study set out to answer?

To develop and validate a simple, accurate, and reproducible colorimetric assay for quantifying angiotensin-I converting enzyme activity in serum.

November 1, 1981Clinical Chemistry

Colorimetry of angiotensin-I converting enzyme activity in serum.

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To develop and validate a simple, accurate, and reproducible colorimetric assay for quantifying angiotensin-I converting enzyme activity in serum.
Coupled an initial cleavage of p-hydroxyhippuryl-L-histidyl-L-leucine by ACE to hippuricase hydrolysis, generating p-hydroxybenzoic acid and glycine.
Catalyzed the oxidative coupling of p-hydroxybenzoic acid with 4-aminoantipyrine using peroxidase and hydrogen peroxide, measuring quinoneimine dye absorbance at 505 nm.
The enzymatic reaction yielded a Michaelis constant (Km) of 0.32 mmol/L with an optimum reaction pH of 8.3.
The colorimetric assay demonstrated a within-run coefficient of variation of 2.93% and correlated closely with the Cushman and Cheung reference method (r = 0.986).

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Implication

Laboratory evaluation demonstrates an accurate colorimetric assay for angiotensin-I converting enzyme in serum, indicating strong agreement with established benchmark methods.

Cite This Study

Kasahara et al. (1981) studied this question.

synapsesocial.com/papers/6a6bb8e978a09e5ab7b3b59c https://doi.org/10.1093/clinchem/27.11.1922

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Implication

Laboratory evaluation demonstrates an accurate colorimetric assay for angiotensin-I converting enzyme in serum, indicating strong agreement with established benchmark methods.

Colorimetry of angiotensin-I converting enzyme activity in serum.

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Discussion

Implication

Cite This Study

Colorimetry of angiotensin-I converting enzyme activity in serum.

Key Points

Discussion

Implication

Cite This Study

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