What question did this study set out to answer?

Investigate how vitamin D status influences the microscopic transit and intracellular localization of calcium across duodenal epithelium.

August 1, 1990Proceedings of the National Academy of SciencesOpen Access

Ion microscopic imaging of calcium transport in the intestinal tissue of vitamin D-deficient and vitamin D-replete chickens: a 44Ca stable isotope study.

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Authors

SCSubhash ChandraManipal Academy of Higher Education CFCurtis S. FullmerCornell University CSChristina SmithUniversity of Washington

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Implication

Animal imaging study reveals accelerated transcellular calcium transport in vitamin D-replete chickens, indicating vitamin D facilitates apical-to-basolateral intestinal movement.

Key Points

Investigate how vitamin D status influences the microscopic transit and intracellular localization of calcium across duodenal epithelium.
Injected 20 mM of 44Ca stable isotope into the duodenal lumen in situ of vitamin D-deficient and vitamin D-replete chickens.
Obtained duodenal tissues at 2.5, 5.0, and 20.0 minutes post-injection for cryosectioning and ion microscopic visualization.
Conducted parallel quantitative absorption experiments using radioactive 47Ca and mapped distribution of endogenous 40Ca and 39K.
At 2.5 minutes, 44Ca was concentrated immediately subjacent to the microvillar membrane in both vitamin D-deficient and D-replete tissues.
At 5.0 and 20.0 minutes, 44Ca remained confined beneath the microvillar membrane in D-deficient tissue, whereas it distributed homogeneously throughout D-replete enterocytes.

Cite This Study

Chandra et al. (1990) studied this question.

synapsesocial.com/papers/6a6bc22fc068f641bcf42c92 https://doi.org/10.1073/pnas.87.15.5715

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Implication

Animal imaging study reveals accelerated transcellular calcium transport in vitamin D-replete chickens, indicating vitamin D facilitates apical-to-basolateral intestinal movement.

Ion microscopic imaging of calcium transport in the intestinal tissue of vitamin D-deficient and vitamin D-replete chickens: a 44Ca stable isotope study.

Discussion

Member takes

Implication

Key Points

Cite This Study

Ion microscopic imaging of calcium transport in the intestinal tissue of vitamin D-deficient and vitamin D-replete chickens: a 44Ca stable isotope study.

Discussion

Member takes

Implication

Key Points

Cite This Study

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