What question did this study set out to answer?

To determine whether gamma interferon-mediated production of nitric oxide inhibits Chlamydia trachomatis proliferation and infectivity in murine McCoy cells.

February 1, 1993Infection and ImmunityOpen Access

Gamma interferon-induced nitric oxide production reduces Chlamydia trachomatis infectivity in McCoy cells

View Full Paper

Ask AI

Bookmark

Authors

JMJeanmarie MayerSpencer Foundation MWMarion L. WoodsThe University of Queensland ZVZdenek VavrinLake City VA Medical Center

Discussion

Member takes

Implication

In vitro study demonstrates that interferon-gamma-induced nitric oxide suppresses Chlamydia trachomatis infectivity in McCoy cells, indicating nitric oxide acts as a key antichlamydial effector.

Key Points

To determine whether gamma interferon-mediated production of nitric oxide inhibits Chlamydia trachomatis proliferation and infectivity in murine McCoy cells.
Primed McCoy cells with recombinant murine IFN-gamma (0 to 50 U/ml) alongside secondary stimuli including Chlamydia trachomatis type H, E. coli lipopolysaccharide, IL-1 alpha, or TNF-alpha.
Quantified infectious chlamydial progeny by measuring inclusion-forming units per milliliter at 48 and 72 hours postinfection.
Inhibited nitric oxide synthase dose-dependently using N-guanidino-monomethyl L-arginine (MLA) to test the mechanism of antichlamydial activity.
IFN-gamma induced nitric oxide production in a dose-dependent manner and reduced C. trachomatis inclusion-forming units by 4 log10 units at 50 U/ml compared with controls at 48 and 72 hours postinfection.
The antichlamydial effect of IFN-gamma was directly proportional to the amount of nitric oxide synthesized.
Addition of the nitric oxide synthase inhibitor MLA reversed the antichlamydial effects of IFN-gamma in a dose-dependent fashion.

Cite This Study

Mayer et al. (1993) studied this question.

synapsesocial.com/papers/6a6bc2f33e46b6df0af32a2f https://doi.org/10.1128/iai.61.2.491-497.1993

View Full Paper

Ask AI

Bookmark

Implication

In vitro study demonstrates that interferon-gamma-induced nitric oxide suppresses Chlamydia trachomatis infectivity in McCoy cells, indicating nitric oxide acts as a key antichlamydial effector.

Gamma interferon-induced nitric oxide production reduces Chlamydia trachomatis infectivity in McCoy cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Gamma interferon-induced nitric oxide production reduces Chlamydia trachomatis infectivity in McCoy cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider