What question did this study set out to answer?

To elucidate the biosynthetic origin and metabolic precursors of the complex thiopeptide antibiotic nosiheptide in Streptomyces actuosus.

August 1, 1988Journal of the American Chemical Society

Biosynthesis of the modified peptide antibiotic nosiheptide in Streptomyces actuosus

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Authors

DHDavid R. HouckMerck & Co., Inc., Rahway, NJ, USA (United States)LCLi Chun. Chen PKPaul J. KellerAmes Research Center

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Implication

Biochemical analysis uncovers the precursor origins of nosiheptide in Streptomyces actuosus, highlighting core enzymatic assembly routes for modified peptide antibiotics.

Key Points

To elucidate the biosynthetic origin and metabolic precursors of the complex thiopeptide antibiotic nosiheptide in Streptomyces actuosus.
Cultured Streptomyces actuosus in the presence of stable isotope-labeled precursors, including carbon-13 and nitrogen-15 labeled amino acids.
Isolated the resulting nosiheptide and analyzed precursor incorporation patterns using carbon-13 and proton nuclear magnetic resonance (NMR) spectroscopy.
Identified the specific amino acid precursors that contribute carbon and nitrogen atoms to the macrocyclic core and modified heterocyclic rings of nosiheptide.
Elucidated the distinct metabolic pathways responsible for constructing the indolic acid residue and central pyridine-containing framework of the antibiotic.

Cite This Study

Houck et al. (1988) studied this question.

synapsesocial.com/papers/6a6bd349c068f641bcf42f5e https://doi.org/10.1021/ja00225a035

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Implication

Biochemical analysis uncovers the precursor origins of nosiheptide in Streptomyces actuosus, highlighting core enzymatic assembly routes for modified peptide antibiotics.

Biosynthesis of the modified peptide antibiotic nosiheptide in Streptomyces actuosus

Discussion

Member takes

Implication

Key Points

Cite This Study

Biosynthesis of the modified peptide antibiotic nosiheptide in Streptomyces actuosus

Discussion

Member takes

Implication

Key Points

Cite This Study

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