Key result
Single nucleotide polymorphisms in the bovine ACSL1 gene were significantly associated with milk production traits and somatic cell score in Chinese Holstein cows (p<0.05).
Why the study?
Improving milk quality is a key challenge, and ACSL1 influences lactation performance, but the genetic effects of ACSL1 single nucleotide polymorphisms on milk production traits required investigation.
Observational (n=992)
p-value: p=<0.05
Polymorphisms in the ACSL1 gene are significantly associated with milk production traits and somatic cell score in Chinese Holstein cows.
Improving the quality of milk is a challenge for zootechnicians and dairy farms across the globe. Long-chain acyl-CoA synthetase 1 (ACSL1) is a significant member of the long-chain acyl-CoA synthetase gene family. It is widely found in various organisms and influences the lactation performance of cows, including fat percentage, milk protein percentage etc. Our study was aimed to investigate the genetic effects of single nucleotide polymorphisms (SNPs) in ACSL1 on milk production traits. Twenty Chinese Holstein cows were randomly selected to extract DNA from their blood samples for PCR amplification and sequencing to identify SNPs of the bovine ACSL1 gene, and six SNPs (5’UTR-g.20523C>G, g.35446C>T, g.35651G>A, g.35827C>T, g.35941G>A and g.51472C>T) were discovered. Then, Holstein cow genotyping (n = 992) was performed by Sequenom MassARRAY based on former SNP information. Associations between SNPs and milk production traits and somatic cell score (SCS) were analyzed by the least-squares method. The results showed that SNP g.35827C>T was in high linkage disequilibrium with g.35941G>A. Significant associations were found between SNPs and test-day milk yield (TDMY), fat content (FC), protein content (PC) and SCS (p < 0.05). Among these SNPs, SNP 5’UTR-g.20523C>G showed an extremely significant effect on PC and SCS (p < 0.01). The SNP g.35446C>T showed a statistically significant effect on FC, PC, and SCS (p < 0.01), and also TDMY (p < 0.05). The SNP g.35651G>A had a statistically significant effect on PC (p < 0.01). The SNP g.35827C>T showed a highly significant effect on TDMY, FC, and SCS (p < 0.01) and significantly influenced PC (p < 0.05). Lastly, SNP g.51472C>T was significantly associated with TDMY, FC, and SCS (p < 0.05). In summary, the pleiotropic effects of bovine ACSL1 for milk production traits were found in this paper, but further investigation will be required on the intrinsic correlation to provide a theoretical basis for the research on molecular genetics of milk quality traits of Holstein cows.
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Liang et al. (2020) conducted an observational in Milk production traits (n=992). ACSL1 gene single nucleotide polymorphisms (SNPs) vs. Alternative alleles/genotypes was evaluated on Test-day milk yield, fat content, protein content, and somatic cell score (p=<0.05). Single nucleotide polymorphisms in the bovine ACSL1 gene were significantly associated with milk production traits and somatic cell score in Chinese Holstein cows (p<0.05).
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