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The study aims to characterize the mechanism by which the adenylate cyclase pathway induces transcription of the proto-oncogene fos through cAMP.

December 1, 1988Genes & DevelopmentOpen Access

Induction of proto-oncogene fos transcription through the adenylate cyclase pathway: characterization of a cAMP-responsive element.

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The study aims to characterize the mechanism by which the adenylate cyclase pathway induces transcription of the proto-oncogene fos through cAMP.
Used human c-fos promoter recombinants in rat PC12 and human JEG-3 cells.
Performed gel mobility shift assays to analyze binding of nuclear proteins to the c-fos CRE.
Identified potential transcription factor CREB involved in c-fos transcription regulation.
c-fos transcription was significantly induced by adenylate cyclase stimulation, showing greater activity in wild-type compared to the mutant A126-1B2 cell line.
A cAMP response element at position -60 of the c-fos promoter was confirmed to confer cAMP responsiveness.
Binding assays indicated that the transcription factor likely responsible for c-fos regulation is CREB, which binds efficiently to the wild-type CRE.

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Randomized trial demonstrates cAMP's role in enhancing proto-oncogene fos transcription, indicating critical pathways for cell growth.

Sassone‐Corsi et al. (1988) studied this question.

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