Key result
An improved multi-electrode surface ECG recording method in adult zebrafish yielded high-quality, reproducible signals that accurately modeled human hyperkalemia, heart injury, and long QT syndrome.
An improved surface ECG recording technique in adult zebrafish provides a reliable and reproducible model for studying human cardiovascular electrophysiology and arrhythmias.
Improved zebrafish ECG method may aid preclinical modeling; leaves open translation to human cardiovascular research.
The adult zebrafish has been used to model the electrocardiogram (ECG) for human cardiovascular studies. Nonetheless huge variations are observed among studies probably because of the lack of a reliable and reproducible recording method. In our study, an adult zebrafish surface ECG recording technique was improved using a multi-electrode method and by pre-opening the pericardial sac. A convenient ECG data analysis method without wavelet transform was also established. Intraperitoneal injection of KCl in zebrafish induced an arrhythmia similar to that of humans, and the arrhythmia was partially rescued by calcium gluconate. Amputation and cryoinjury of the zebrafish heart induced ST segment depression and affected QRS duration after injury. Only cryoinjury decelerated the heart rate. Different changes were also observed in the QT interval during heart regeneration in these two injury models. We also characterized the electrocardiophysiology of breakdance zebrafish mutant with a prolonged QT interval, that has not been well described in previous studies. Our study provided a reliable and reproducible means to record zebrafish ECG and analyse data. The detailed characterization of the cardiac electrophysiology of zebrafish and its mutant revealed that the potential of the zebrafish in modeling the human cardiovascular system exceeds expectations.
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Liu et al. (2016) studied Cardiovascular disease modeling. Multi-electrode surface ECG recording with pre-opened pericardial sac vs. Conventional ECG recording methods was evaluated on ECG signal quality and interval measurements. An improved multi-electrode surface ECG recording method in adult zebrafish yielded high-quality, reproducible signals that accurately modeled human hyperkalemia, heart injury, and long QT syndrome.
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