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July 31, 2026Discover Industrial Chemistry and MaterialsOpen Access

Sustainable bioconversion of waste cooking oil to biodiesel using an immobilized Pseudomonas aeruginosa lipase

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Authors

KGK. R. GaneshTNT. M. NingarajuAPAnitha Peter

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Overview

Randomized trial shows effective biodiesel production from waste cooking oil, indicating environmental benefits.

Key Points

  • This research aims to explore the sustainable conversion of waste cooking oil to biodiesel using immobilized lipase from Pseudomonas aeruginosa.
  • Lipase was cloned, expressed, purified, and immobilized in calcium-alginate beads.
  • Transesterification was performed with optimized conditions including molar ratio and temperature.
  • The activity profile was assessed across various pH levels and temperatures.
  • Maximum specific activity of the enzyme reached 6,595.7 U·mg − 1 after purification.
  • Yield of fatty acid methyl esters was 68.43% under optimized conditions.
  • Enzyme exhibited activity at pH 8.0 with an activation energy of 51.3 kJ·mol − 1.

Cite This Study

Ganesh et al. (2026) studied this question.

synapsesocial.com/papers/6a6c46c0747664a1aa73bbbahttps://doi.org/10.1007/s44508-026-00016-9
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Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties2013 · 485 citations
  2. 2Lipase-Catalyzed Esterification2000 · 179 citations
  3. 3Enzyme Immobilization on Inorganic Surfaces for Membrane Reactor Applications: Mass Transfer Challenges, Enzyme Leakage and Reuse of Materials2018 · 203 citations