Identification of key target genes and pathway analysis in nonalcoholic fatty liver disease via integrated bioinformatics analysis

Figure 1. The hierarchically clustering analysis of screened differentially expressed RNAs (DERs). Left: log2FC-log10 (FDR) volcano map for GSE83452 using the significant DERs. Blue and red dots indicate significant DERs. The horizontally dashed line indicates FDR < 0.05. Two vertical lines indicate |Log2FC| > 0.5. A: Baseline time points; B: 1-year follow-up time points. Right: Two-way hierarchically clustered heat map for GSE83452 using the DERs. Red: upregulated DERs. Blue: downregulated DERs. C: Baseline time points; D: 1-year follow-up time points.

Figure 2. Authentication of overlapping DERs in the GSE83452 datasets via Venn diagrams software. Blue represents DERs of the baseline time points group. Yellow represents DERs of the 1-year follow-up time points group.

Figure 3. The lncRNA–miRNA–mRNA ceRNA network. Squares, triangles, and circles represent lncRNA, miRNA, and mRNA, respectively. Green and red dots indicate the significantly downregulated DERs at both baseline and 1-year follow-up time points, and the white dots indicate DERs whose expression difference direction has changed.

Figure 4. Gene–drug connection network. Squares represent drug molecules, circles represent genes, and green and red dots represent the significantly downregulated DERs at both baseline and 1-year follow-up time points.