Effects of Yiqi Huoxue Decoction on Post-Myocardial Infarction Cardiac Nerve Remodeling and Cardiomyocyte Hypertrophy in Rats

Figures

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  Figure 1. (a) Echocardiogram and cardiac function of rats in each group 28 days later (N = 9). ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group. (b) Histopathological hematoxylin and eosin staining of infarct marginal area tissue of rats with myocardial infarction in each group (scale bar = 200 μm).

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  Figure 2. (a) Immunohistochemical staining to observe the TH staining positive sympathetic fibers in the infarct marginal zone of myocardial infarction rats (scale bar = 100 μm). (b) The expression of TH in the infarct marginal area tissue of each group. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group. (c) The effect of YQHX on the expression of GAP43, NGF, and Sema-3A. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group.

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  Figure 3. The expression of nerve factors in stellate ganglia. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group.

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  Figure 4. The expression of NPY in the infarct marginal zone tissue and serum of each group. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group.

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  Figure 5. (a) Observation of the cross section of cardiomyocytes stained with histopathological hematoxylin and eosin and the effect of YQHX on the cross-sectional area, average diameter, and perimeter of cardiomyocytes (scale bar = 50 μm). (b) The effect of YQHX on the expression of ANP and β-MHC protein in the marginal zone of myocardial infarction in rats. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group.

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  Figure 6. The effect of YQHX on ERK1/2 protein and its phosphorylation. ##P < 0.01, vs. the sham operation group; ∗∗P < 0.01, vs. MI model group.

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  Figure 7. (a) The effect of YQHX on the levels of SOD, MDA, and LDH in cell supernatant. ##P < 0.01, vs. the C group; ∗∗P < 0.01, vs. the M group. (b) Morphological changes of cardiomyocytes in each group (scale bar = 100 μm). (c) Hoechst 33258 staining showed the apoptosis of each group (scale bar = 100 μm). (d) NGF protein expression in each group of cells. ##P < 0.01, vs. the C group; ∗∗P < 0.01, vs. the M group.

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  Figure 8. The expression of ERK1/2 signaling pathway related proteins in each group of cells. ##P < 0.01, vs. the C group; ∗P < 0.05, vs. the M group; ∗∗P < 0.01, vs. the M group.