Genotyping of Heterogenous Osteopetrotic Mice Using Microsatellite Markers.

The aim of this study was to elucidate the three genotypes (op/op, op/+ and +/+) of heterogenous osteopetrotic mice using microsatellite markers. The genotyping analysis was performed on genetic crosses using B6C3Fe-op/+ and A/WySnJ mice. In this study, we used five MIT markers, D3Mit11, 75, 101, 102 and 189 that are closely linked to the op locus and which segregate polymorphic variants in mating between B6C3Fe-op/+ and A/WySnJ mice, and the homozygous op/op F3 mice were obtained by F2 mice mating. From the results of F3 genotyping, it was possible to determine the genotype of the op gene without a complicated molecular process. Histopathological studies revealed that the F3-op/op mice showed similar femur bone structures to those of B6C3Fe-op/op mice. Based on these findings, it is suggested that genotyping of heterogenous osteopetrotic mice using microsatellite markers is a powerful technique for investigating the mechanism of osteoclast development and genetic studies of osteopetrosis.