What question did this study set out to answer?

To evaluate the presence and molecular forms of C-type natriuretic peptide (CNP) in cultured human vascular endothelial cells and circulating human plasma.

October 1, 1992AJP Heart and Circulatory Physiology

Presence of C-type natriuretic peptide in cultured human endothelial cells and plasma

Q: What is the clinical evidence from this study?

Study design: Other. Intervention: C-type natriuretic peptide (CNP) assessment. Primary outcome: Presence and concentration of CNP immunoreactivity.

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Key result

C-type natriuretic peptide immunoreactivity was present in cultured human aortic endothelial cells as CNP-53 and in human plasma (n=22) at 6.5 +/- 0.2 pg/ml as CNP-22.

Authors

ASA. J. StingoMayo Clinic ACAlfredo L. ClavellHeart Failure / Cardiomyopathy DHDenise M. HeubleinMahindra and Mahindra Limited (India)

Discussion

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Implication

Does not alter practice; extends animal data to human endothelium and leaves open physiological roles.

Key Points

To evaluate the presence and molecular forms of C-type natriuretic peptide (CNP) in cultured human vascular endothelial cells and circulating human plasma.
Assessed cultured human aortic endothelial cells for CNP immunoreactivity using immunohistochemical staining, radioimmunoassay, and gel permeation chromatography.
Quantified and characterized circulating CNP in human plasma samples (N=22) using specific radioimmunoassay and gel permeation chromatography.
Cultured aortic endothelial cells demonstrated CNP immunoreactivity corresponding to the higher molecular weight CNP-53 form.
Human plasma contained CNP immunoreactivity at a concentration of 6.5 ± 0.2 pg/ml, which aligned with the lower molecular weight CNP-22 form.

PICO

Population

22 human plasma samples and cultured human aortic endothelial cells analyzed for C-type natriuretic peptide.

Exposure / Comparator

C-type natriuretic peptide (CNP) assessment

Primary Outcome

Presence and concentration of CNP immunoreactivity

Cite This Study

Stingo et al. (1992) studied this question. C-type natriuretic peptide (CNP) assessment was evaluated on Presence and concentration of CNP immunoreactivity. C-type natriuretic peptide immunoreactivity was present in cultured human aortic endothelial cells as CNP-53 and in human plasma (n=22) at 6.5 +/- 0.2 pg/ml as CNP-22.

synapsesocial.com/papers/6a6cd155f44fa9f079db6559 https://doi.org/10.1152/ajpheart.1992.263.4.h1318

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Presence of C-type natriuretic peptide in cultured human endothelial cells and plasma

Discussion

Member takes

Implication

Key Points

PICO

Cite This Study

Presence of C-type natriuretic peptide in cultured human endothelial cells and plasma

Discussion

Member takes

Implication

Key Points

PICO

Cite This Study

Also Consider

Also Consider