Key result
Using a 0.1-Hz high-pass filter provided more reliable field potential waveforms and concentration-response curves for QT prolongation assessment in iPS cell-derived cardiomyocytes compared to 1-Hz.
Population
Human induced pluripotent stem (iPS) cell-derived cardiomyocyte sheets
Comparison
High-pass filter frequency of 0.1 Hz and varying… vs High-pass filter (HPF) frequency of 1 Hz
Design
Preclinical
Authors
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Optimizing MEA conditions in iPS-CMs may enhance FP reliability; leaves open translation to proarrhythmia prediction pending validation.
A 0.1-Hz high-pass filter provides more reliable field potential data than a 1-Hz filter for assessing proarrhythmic potential in iPS cell-derived cardiomyocytes.
Asakura et al. (2015) studied this question. High-pass filter (HPF) frequency (0.1 or 1Hz) and cell plating density was evaluated on Field potential duration (FPD) and waveform characteristics. Using a 0.1-Hz high-pass filter provided more reliable field potential waveforms and concentration-response curves for QT prolongation assessment in iPS cell-derived cardiomyocytes compared to 1-Hz.
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