Key result
Sustained ligand-activated preconditioning via 5 days of morphine treatment reduced post-ischemic cellular death by >75% and induced a unique transcriptional profile repressing inflammation.
Why the study?
Does sustained ligand-activated preconditioning with morphine alter the transcriptional profile and improve ischemia-reperfusion tolerance in mouse hearts?
Population
Male C57Bl/6 mice
Comparison
75 mg morphine pellet implanted for 5 days to… vs Placebo pellet implanted for 5 days
Design
Preclinical
Follow-up
5 days
Authors
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Hypothesis-generating for SLP mediators in myocardium; leaves open translation to clinical cardioprotection.
Does sustained ligand-activated preconditioning with morphine alter the transcriptional profile and improve ischemia-reperfusion tolerance in mouse hearts?
Effect estimate: >75% reduction
p-value: p=<0.05
Sustained opioid receptor agonism induces cardioprotection against ischemia-reperfusion injury via a unique transcriptional profile involving repression of inflammatory genes and upregulation of sarcomeric and natriuretic peptide genes.
Ashton et al. (2013) studied Myocardial ischemia-reperfusion injury (n=28). Sustained ligand-activated preconditioning (morphine) vs. Placebo pellet was evaluated on Post-ischemic LDH efflux (cellular death/damage) (>75% reduction, p=<0.05). Sustained ligand-activated preconditioning via 5 days of morphine treatment reduced post-ischemic cellular death by >75% and induced a unique transcriptional profile repressing inflammation.
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