Helium Induces Preconditioning in Human Endothelium In Vivo

Kirsten Smit; Gezina T.M.L. Oei; Daniel Brevoord; Erik S.G. Stroes; Rienk Nieuwland; W. Schlack; Markus W. Hollmann; Nina C. Weber; Benedikt Preckel

doi:10.1097/aln.0b013e3182751300

December 5, 2012AnesthesiologyOpen Access

Helium Induces Preconditioning in Human Endothelium In Vivo

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Key Points

To determine whether inhalation of the noble gas helium prevents postischemic endothelial dysfunction in humans in vivo and to examine the involvement of nitric oxide synthase, inflammatory cytokines, and microparticles.
In a trial of 58 healthy volunteers, 50 subjects were randomized to five groups (time control, ischemia–reperfusion [I/R], ischemic preconditioning, early helium preconditioning [He-EPC], or late helium preconditioning at 24 h), alongside an additional cohort of 8 subjects receiving the eNOS blocker L-NMMA.
Helium was delivered as a 79% helium/21% oxygen mixture in three 5-minute cycles alternating with air breathing before 20 minutes of forearm ischemia induced by cuff occlusion at 200 mmHg.
Vascular endothelial function was measured by bilateral venous occlusion plethysmography during intrabrachial infusion of acetylcholine and sodium nitroprusside, combined with venous blood assays for cytokines, adhesion molecules, and cell-derived microparticles.
Forearm I/R blunted the dose-dependent vasodilatory response to acetylcholine (P = 0.001), confirming acute endothelial dysfunction, while the response to sodium nitroprusside remained intact.
He-EPC preserved acetylcholine-induced vasodilation (P = 0.581 vs. baseline), and late helium preconditioning 24 hours prior similarly maintained endothelial function (P = 0.165), achieving protection comparable to ischemic preconditioning (P = 0.657).
Inhibition of eNOS with L-NMMA reduced baseline forearm blood flow by 61 ± 8% but failed to block helium-mediated protection (P = 0.720), while systemic levels of adhesion molecules, cytokines, and microparticles showed no significant alterations across groups.

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Implication

Demonstrates that helium inhalation prevents ischemia-reperfusion endothelial dysfunction in healthy adults, suggesting potential as a safe vascular protective agent.

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Smit et al. (2012) studied this question.

synapsesocial.com/papers/6a6d050878a11c550e07c4b5 https://doi.org/10.1097/aln.0b013e3182751300

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Helium Induces Preconditioning in Human Endothelium In Vivo

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Helium Induces Preconditioning in Human Endothelium In Vivo

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