What question did this study set out to answer?

To evaluate the catalytic capacity of imine reductases and investigate the unexpected reduction of bicyclic iminium substrates in the presence of glucose dehydrogenase.

July 19, 2017ChemBioChem

Extended Catalytic Scope of a Well‐Known Enzyme: Asymmetric Reduction of Iminium Substrates by Glucose Dehydrogenase

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Authors

SRSebastian RothUniversity of Freiburg APAndreas PrägUniversity of Freiburg CWCindy WechslerUniversity of Freiburg

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Implication

In vitro experiment demonstrates catalytic reduction of iminium substrates by glucose dehydrogenase, indicating unexpected enzyme promiscuity.

Key Points

To evaluate the catalytic capacity of imine reductases and investigate the unexpected reduction of bicyclic iminium substrates in the presence of glucose dehydrogenase.
Assayed a panel of NADP(H)-dependent imine reductases against a set of bicyclic iminium compounds.
Performed negative control assays lacking imine reductases to assess background reactivity with glucose dehydrogenase from different origins.
Determined substrate conversion rates and product enantiomeric excess using 1H NMR and gas chromatography.
All evaluated imine reductases successfully converted the bicyclic iminium substrates into chiral amines.
Negative control reactions lacking imine reductases revealed catalytic iminium reduction mediated directly by glucose dehydrogenase.
Glucose dehydrogenases from different origins achieved substrate conversion exceeding 50% and enantioselectivity up to >99% enantiomeric excess.

Cite This Study

Roth et al. (2017) studied this question.

synapsesocial.com/papers/6a6d22e7e36a167817dfdfff https://doi.org/10.1002/cbic.201700261

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Implication

In vitro experiment demonstrates catalytic reduction of iminium substrates by glucose dehydrogenase, indicating unexpected enzyme promiscuity.

Extended Catalytic Scope of a Well‐Known Enzyme: Asymmetric Reduction of Iminium Substrates by Glucose Dehydrogenase

Discussion

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Implication

Key Points

Cite This Study

Extended Catalytic Scope of a Well‐Known Enzyme: Asymmetric Reduction of Iminium Substrates by Glucose Dehydrogenase

Discussion

Member takes

Implication

Key Points

Cite This Study

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