Key result
Obese rats fed a high-fat diet showed impaired insulin- and acetylcholine-stimulated IRS-1/PI 3-kinase signaling in the aorta, leading to reduced eNOS activation and lower eNOS protein levels.
Population
Rat aorta (normal and obese rats on high-fat diet)
Comparison
Acetylcholine and insulin stimulation… vs Normal rats vs obese rats; unblocked vs blocked…
Design
Preclinical
Authors
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ACh-JAK2-IRS-1 signaling in rat aorta is hypothesis-generating; leaves open relevance to human endothelial dysfunction in diabetes.
Obesity induces both insulin and acetylcholine resistance in rat aorta via impairment of a common IRS-1/PI 3-kinase signaling pathway that controls eNOS activity.
Zecchin et al. (2007) studied Obesity. High-fat diet was evaluated on IRS-1/PI 3-kinase pathway activation and eNOS levels. Obese rats fed a high-fat diet showed impaired insulin- and acetylcholine-stimulated IRS-1/PI 3-kinase signaling in the aorta, leading to reduced eNOS activation and lower eNOS protein levels.
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