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August 16, 2017Journal of Clinical Laboratory AnalysisOpen Access

Finding the candidate sequence variants for diagnosis of hypertrophic cardiomyopathy in East Slovak patients

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Key result

Genetic sequencing of six cardiomyopathy genes in 23 patients with hypertrophic cardiomyopathy identified 43 sequence variants, of which 58.14% were novel and 11 were potentially pathogenic.

Why the study?

What are the candidate sequence variants in selected cardiomyopathy genes in East Slovak patients with hypertrophic cardiomyopathy?

Population

23 unrelated patients with hypertrophic cardiomyopathy and 25 healthy controls from East Slovakia

Comparison

Conventional capillary-based Sanger sequencing… vs Healthy controls

Design

Case-control

Authors

MZMichaela ZigováJBJarmila BernasovskáIBIveta Boroňová

Discussion

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Overview

May inform regional HCM genetic screening; leaves open pathogenicity confirmation and broader applicability.

Study Design

Type

Case-Control (n=48)

Structured PICO

What are the candidate sequence variants in selected cardiomyopathy genes in East Slovak patients with hypertrophic cardiomyopathy?

P
Population
23 unrelated patients with hypertrophic cardiomyopathy (mean age 58.1 years) and 25 healthy controls from East Slovakia evaluated for candidate sequence variants.
E
Exposure
Conventional capillary-based Sanger sequencing of selected exons of six cardiomyopathy genes (MYBPC3, MYH7, NEBL, SCN5A, TNNI3, TNNT2)
C
Comparator
Healthy controls
O
Outcome
Identification of candidate sequence variants and potentially pathogenic genetic alterationssurrogate

The study identified novel and known sequence variants, particularly in exon 23 of the MYH7 gene, as potential candidate genetic variants for hypertrophic cardiomyopathy in East Slovak patients.

Cite This Study

Zigová et al. (2017) conducted a case-control in Hypertrophic cardiomyopathy (n=48). Sequence variants in six cardiomyopathy genes (MYBPC3, MYH7, NEBL, SCN5A, TNNI3, TNNT2) vs. Healthy controls was evaluated on Presence of candidate sequence variants in selected exons of six cardiomyopathy genes. Genetic sequencing of six cardiomyopathy genes in 23 patients with hypertrophic cardiomyopathy identified 43 sequence variants, of which 58.14% were novel and 11 were potentially pathogenic.

synapsesocial.com/papers/6a6d3aaaf44fa9f079db963ahttps://doi.org/10.1002/jcla.22303
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Targeted next-generation sequencing helps to decipher the genetic and phenotypic heterogeneity of hypertrophic cardiomyopathy2016 · 37 citations
  2. 2Defining the genetic architecture of hypertrophic cardiomyopathy: re-evaluating the role of non-sarcomeric genes2016 · 208 citations
  3. 3Genetic advances in sarcomeric cardiomyopathies: state of the art2015 · 238 citations
  4. 4Early-Onset Hypertrophic Cardiomyopathy Mutations Significantly Increase the Velocity, Force, and Actin-Activated ATPase Activity of Human β-Cardiac Myosin2016 · 94 citations
  5. 5New Perspectives on the Prevalence of Hypertrophic Cardiomyopathy2015 · 1,426 citations