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August 1, 2026Molecular Reproduction and DevelopmentOpen Access

Epididymosome‐Supplemented Extender Induces Changes in Morpho‐Functional Traits and microRNA Levels of Post‐Thaw Sperm and Improves Embryo Developmental Potential

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Authors

LHLaura Gabrielli HaupenthalMAMaria Alice de AlmeidaCPCibele M. Prado

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Overview

Randomized trial reveals that epididymosome supplementation improves sperm quality and embryo development, indicating enhanced fertility potential.

Key Points

  • To assess the impact of epididymosomes on post-thaw sperm quality and fertility potential after cryopreservation.
  • Epididymal sperm from six bulls (n=6) were cryopreserved with or without PKH67-labeled epididymosomes.
  • Sperm quality was evaluated post-thaw for motility, membrane integrity, and microRNA levels using flow cytometry and microscopy.
  • In vitro embryo production (IVEP) was performed to assess developmental potential post-cryopreservation.
  • EpEVs-sperm showed significantly higher motility and plasma membrane integrity than Control-sperm (p<0.05).
  • 28 unique microRNAs were detected in epEVs-sperm, indicating enhanced molecular quality.
  • IVEP with epEVs-sperm resulted in a higher rate of hatched blastocysts compared to Control.

Cite This Study

Haupenthal et al. (2026) studied this question.

synapsesocial.com/papers/6a6d98aae258b358b3c6c5edhttps://doi.org/10.1002/mrd.70138
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