What question did this study set out to answer?

To examine how oleic acid influences pyruvate oxidation and gluconeogenic flux in isolated perfused livers from fasted rats.

September 1, 1967European Journal of BiochemistryOpen Access

Effect of Oleic Acid on the Oxidation and Gluconeogenesis from 1‐14CPyruvate in the Perfused Rat Liver

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Authors

HTHannes TEUFELMünchen Klinik Schwabing LMLawrence A. MenahanMarquette University JSJoseph C. ShippUniversity of Nebraska at Omaha

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Implication

Ex vivo perfusion study demonstrates oleic acid enhances gluconeogenesis by inhibiting pyruvate oxidation in rat liver, suggesting fatty acids optimize glucose synthesis efficiency.

Key Points

To examine how oleic acid influences pyruvate oxidation and gluconeogenic flux in isolated perfused livers from fasted rats.
Perfused isolated livers from fasted rats with [1-14C]pyruvate in the presence or absence of oleic acid.
Tracked carbon balances, 14CO2 production, liver glycogen, and medium [14C]glucose specific activity, and assessed the comparative effect of glucagon on pyruvate versus glycerol.
Oleic acid stimulated gluconeogenesis, increasing medium glucose, liver glycogen, and [14C]glucose specific activity.
Oleic acid altered the carbon utilization ratio, requiring 2 moles of pyruvate per mole of synthesized glucose compared to a 4:1 ratio without fatty acid.
Pyruvate oxidation was markedly suppressed by oleic acid as measured by 14CO2 output, while glucagon enhanced glucose synthesis from pyruvate but not glycerol.

Cite This Study

Teufel et al. (1967) studied this question.

synapsesocial.com/papers/6a6d9cbb75498292b707ebc0 https://doi.org/10.1111/j.1432-1033.1967.tb00124.x

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Implication

Ex vivo perfusion study demonstrates oleic acid enhances gluconeogenesis by inhibiting pyruvate oxidation in rat liver, suggesting fatty acids optimize glucose synthesis efficiency.

Effect of Oleic Acid on the Oxidation and Gluconeogenesis from 1‐14CPyruvate in the Perfused Rat Liver

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Implication

Key Points

Cite This Study

Effect of Oleic Acid on the Oxidation and Gluconeogenesis from 1‐14CPyruvate in the Perfused Rat Liver

Discussion

Member takes

Implication

Key Points

Cite This Study

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