What question did this study set out to answer?

This research aims to describe how l-methionine is transported into Escherichia coli cells and its metabolic fate.

January 1, 1974Journal of BacteriologyOpen Access

Transport Systems for l -Methionine in Escherichia coli

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Randomized trial demonstrates l-methionine transport mechanisms in Escherichia coli, highlighting its uptake and energy dependency.

This research aims to describe how l-methionine is transported into Escherichia coli cells and its metabolic fate.
Examined methionine uptake at varying concentrations and temperatures.
Identified multiple transport systems with different kinetic properties.
Studied the effect of inhibitors on the uptake process.
High-affinity transport system showed K(T) = 7.5 x 10(-8) M, V(T) = 200 pmol/mL/min.
Low-affinity transport system demonstrated K(T) = 40 x 10(-6) M, V(T) = 1,550 pmol/mL/min.
Uptake of l-methionine was energy-dependent, sourced from glycolysis or oxidative phosphorylation.

Robert J. Kadner (1974) studied this question.

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