What question did this study set out to answer?

To determine the role of cell adherence and aggregation in endotoxin-mediated polymorphonuclear leukocyte (PMN) activation and compare these effects with formylated chemotactic peptide stimulation.

February 1, 1983The Journal of ImmunologyOpen Access

Granulocyte activation by endotoxin. II. Role of granulocyte adherence, aggregation, and effect of cytochalasin B, and comparison with formylated chemotactic peptide-induced stimulation.

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To determine the role of cell adherence and aggregation in endotoxin-mediated polymorphonuclear leukocyte (PMN) activation and compare these effects with formylated chemotactic peptide stimulation.
Assessed PMN adhesiveness, enzyme release, hexose monophosphate shunt activity, and superoxide production under stationary culture and suspension conditions following endotoxin exposure.
Tested PMN aggregation responses and activation pathways in the presence or absence of cytochalasin B and following challenge with formylated chemotactic peptides.
Endotoxin elicited marked increases in PMN adhesiveness, enzyme release, hexose monophosphate shunt activity, and superoxide production in stationary dishes, whereas no activation occurred in suspension.
Unlike formylated chemotactic peptides, endotoxin induced no cellular aggregation with or without cytochalasin B, while combined exposure in stationary conditions produced additive stimulatory effects.

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Implication

Experimental study reveals that endotoxin stimulates granulocytes exclusively during surface adherence rather than in suspension, indicating surface contact is vital for immune activation.

Cite This Study

Dahinden et al. (1983) studied this question.

synapsesocial.com/papers/6a6df21026770c2b8ddfb281 https://doi.org/10.4049/jimmunol.130.2.863

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Implication

Experimental study reveals that endotoxin stimulates granulocytes exclusively during surface adherence rather than in suspension, indicating surface contact is vital for immune activation.

Granulocyte activation by endotoxin. II. Role of granulocyte adherence, aggregation, and effect of cytochalasin B, and comparison with formylated chemotactic peptide-induced stimulation.

Key Points

Discussion

Implication

Cite This Study

Granulocyte activation by endotoxin. II. Role of granulocyte adherence, aggregation, and effect of cytochalasin B, and comparison with formylated chemotactic peptide-induced stimulation.

Key Points

Discussion

Implication

Cite This Study

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